Product Properties Synonyms CD154;TNFSF5IMD3;CD40LG;CD40L Source Human CD40 Ligand/TNFSF5 Protein is expressed from E.coli with His tag at the C-terminus. It contains Gln114-Leu261.[Accession | P29965] Molecular Weight The protein has a predicted MW of 17.15 kDa same as SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human CD40 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.7μg/ml determined by ELISA. Formulation Supplied as 0.22 μm filtered solution in PBS, 10% glycerol (pH 7.4). Storage The product should be stored at -85~-65℃ for 1 year from date of receipt. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.