Product Properties Property Name Property Details Alternative Names CD47,OA3,CD47 molecule,CD47 glycoprotein Species Human Expression Sequence Gln19-Pro139 Accession Q08722-1 Tag No Tag Expression System HEK293 Molecular Weight The protein has a predicted MW of 13.72 kDa. Due to glycosylation, the protein migrates to 35-48 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human CD47, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.24μg/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. For initial use, aliquot the protein to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin levels, and high batch-to-batch consistency Storage Store at -25 to -15°C; valid for 1 year upon receipt.After reconstitution, store under sterile conditions at -85 to -65°C; valid for 3 months.
