Product Properties Alternative Names IL23 alpha&IL12 beta ,IL23 alpha&IL12 β,IL12 beta,IL23 alpha Species Human Expression Sequence Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta) Accession Q9NPF7(IL-23 alpha)&P29460(IL-12 beta) Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 19.77 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 23 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human IL-23 alpha&IL-12 beta, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
