Product Propertie Alternative Names TGF beta1,TGFB1,DPD1,CEDLAP Species Human Expression Sequence Leu30-Ser390(C33S) Accession P01137 Tag N-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa(LAP) / 13-15 kDa(Mature TGF Beta 1) kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human Latent TGF beta 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA. 【ELISA Data】 Immobilized Human Latent TGF beta 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 6.6ug/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
