Human NCAM-1/CD56 Protein, His Tag

Yeasen - Leading Innovation in Molecular Enzymes and Reagents Verified independent store

→

Product Propertie Alternative Names NCAM1,NCAM,NCAM-1,N-CAM-1 Species Human Expression Sequence Leu20-Gly718 Accession P13591-2 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 78.48 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Human NCAM-1, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 5.1 ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months. Show more