Product Propertie Alternative Names GGF,HRGA,NRG1,Pro-NRG1 Species Human Expression Sequence Ser2-Lys246 Accession Q02297-6 Tag N-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 54.00 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE,> 90% as determined by HPLC Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human Her4, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
