Human TIE2 Protein, His Tag

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Product Propertie Alternative Names Tie-2, Angiopoietin-1 receptor, TEK, VMCM, VMCM1, CD202b Species Human Expression Sequence Ala23-Leu748 Accession AAA61139.1 Tag C-His Expressio n System HEK293 Molecular Weight The protein has a predicted MW of 82.09 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【SPR Data】Human TIE2, His Tag immobilized on CM5 Chip can bind Human ANGPT2, His Tag with an affinity constant of 2.38 μM as determined in SPR assay . Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months. Show more