Product Properties Alternative Names Alkaline Phosphatase, ALP-1, Alkaline phosphatase, placental-like, GCAP, PLAP-like, ALPG, ALPPL, ALPPL2 Species Human Expression Sequence Ile20-Asp503 Accession P10696 Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE,> 90% as determined by HPLC Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 23.4ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
