Biotinylated Human CEACAM-5/CD66e Protein, His-Avi Tag

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Product Properties Alternative Names CEACAM-5, CD66e, CEA, Meconium antigen 100, Carcinoembryonic, CEACD66e, DKFZp781M2392 Species Human Expression Sequence Lys35-Ala685 Accession P06731-1 Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 74.23 kDa. Due to glycosylation, the protein migrates to 110-150 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Biotinylated Human CEACAM-5, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months. Show more