Biotinylated Human DLL3 Domain (311-479) Protein, His-Avi Tag

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Product Properties Alternative Names Delta3, DLL3, Pudgy, SCDO1, SCDO1delta3 Species Human Expression Sequence Val311-Ala479 Accession Q9NYJ7-1 Tag C-His-Avi Expression System HEK2 93 Molecular Weight The protein has a predicted MW of 21.20 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Biotinylated Human DLL3 Domain (311-479) ,His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months. Show more