Biotinylated Human IL-10 R alpha Protein, His-Avi Tag

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Alternative Names IL-10RA, IL10RA, IL10R, IL-10R1, IL-10R subunit 1, CDw210a, HIL-10R, IBD28, IL-10 R alpha Species Human Expression Sequence His22-Asn235 Accession Q13651 Tag C-Hi s-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 42-60 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human IL-10, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.0ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months. Show more