Product Properties Alternative Names Secretogranin-3, Secretogranin III, SCG3, SGIII, UNQ2502,PRO5990 Species Cynomolgus Expression Sequence Phe20-Leu468 Accession I7G9U8 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 52.14 kDa. Due to glycosylation, the protein migrates to 58-70 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus SG3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 2 years after receipt of the product. After reconstitution, store under sterile conditions at -85 to -65°C. The shelf life is 3 months.
