Product Properties Alternative Names APC1 protein, Cachectin, DIF, TNF, TNFalpha, TNFATNF, TNFSF1A, TNFSF2, TNFA, TNFα, DIF, TNFSF2,TNF-alpha Species Cynomolgus Expression Sequence Val77-Leu233 Accession P79337 Tag N-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 18.35 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus TNF alpha, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA . Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
