BiotinylatedCynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) , His Tag

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Product Properties Alternative Names CD3e, CD3E, T3E, FLJ18683, TCRE, CD3-epsilon, IMD18 Species Cynomolgus Expression Sequence Gln22-Asp117 Accession Q95LI5 Tag C-His Expression S ystem HEK293 Molecular Weight The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 13-16 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Anti-CD3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months. Show more