Product Properties Alternative Names TL1A, VEGI-251, TNFSF15, TL1, VEGI, VEGI192A, TNF superfamily member 15 Species Cynomolgus,Rhesus macaque Expression Sequence Leu72-Leu251 Accession A0A2K5UA22 Tag N-monomeric Fc Expression System HEK293 Molecular Weight The protein has a predicted MW of 44.82 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE,> 90% as determined by HPLC Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Mouse DR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque TNFSF15, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA. 【ELISA Data】 Immobilized Cynomolgus/Rhesus macaque TNFSF15, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
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