Product Properties Alternative Names SCFR, PBT, CD117, ckit, c-kit, KIT Species Mouse Expression Sequence Ser25-Pro523 Accession P05532-2 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 56.63 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【SPR Data】Mouse CD117, His Tag captured on CM5 Chip via anti-his antibody can bind Human SCF, No Tag with an affinity constant of 14.02 μM as determined in SPR assay . Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
