Product Properties Alternative Names PDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, B7DC, bA574F11.2, Btdc Species Mouse Expression Sequence Leu20-Trp221 Accession Q9WUL5 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 23.92 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Mouse PD-L2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human PD-1, hFc Tag with the EC50 of 0.32μg/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
