Rhesus macaque IL-18 R1/CD218a Protein, hFc Tag

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Product Properties Alternative Names CD218a, IL18Ra, IL18R1, IL1RRP, IL-1 R5, IL-1 Rrp, IL-18 R alpha, IL-18 R1, IL-18Ra Species Rhesus macaque Expression Sequence Cys22-Gly329 Acc ession F7H8F9 Tag C-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 61.96 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Rhesus macaque IL-18 R1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 0.42μg/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months. Show more