Although platelets are best known for their role in hemostasis, they are also crucial in development, host defense, inflammation, and tissue repair . Many of these roles are regulated by the immune-like receptors glycoprotein VI (GPVI) and C-type lectin receptor 2 (CLEC-2), which signal through an immunoreceptor tyrosine-based activation motif (ITAM). Specifications Cat.No. 96263ES25 / 96263ES60 / 96263ES76 Size 25 μg / 100 μg / 500 μg Components Name 96263ES25 96263ES60 96263ES76 Recombinant Cynomolgus GPVI Protein,His Tag,Ultra Low Endotoxin 25 μg 100 μg 500 μg Performance parameters Synonyms GPVI;Gp6;Glycoprotein 6;MGC138168 Species Cynomolgus Expression Sequence Gln21-Asn251 Accession XP_005590417.2 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 26.42 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. Purity > 95% as determined by SDS-PAGE and SEC-HPLC Endotoxin Less than 0.01 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological activity ELISA Data 1:Immobilized Cynomolgus GPVI, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA . Reconstitution Method Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. Storage Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves,for your safety. Documents 96263_Manual_HB2026515
