IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins . It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. Specifications Cat.No. 96267ES25 / 96267ES60 / 96267ES76 Size 25 μg / 100 μg / 500 μg Components Name 96267ES25 96267ES60 96267ES76 Recombinant Cynomolgus IL-1R3/IL-1 RAcP Protein,His Tag,Ultra Low Endotoxin 25 μg 100 μg 500 μg Performance parameters Synonyms IL1RAP;IL-1RAP;C3orf13;IL-1 R3;IL-1 RAcP Species Cynomolgus Expression Sequence Ser21-Cys362 Accession G7NYP7 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 40.49 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. Purity > 95% as determined by SDS-PAGE and SEC-HPLC Endotoxin Less than 0.01 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological activity ELISA Data 1:Immobilized Cynomolgus IL-1R3, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA . Reconstitution Method Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. Storage Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves,for your safety. Documents 96267_Manual_HB2026515
