GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site . Binding of eRF1, eRF3, and GTP to pretermination complexes first induces a structural rearrangement that is manifested as a 2 nucleotide forward shift of the toeprint attributed to pretermination complexes that leads to GTP hydrolysis followed by rapid hydrolysis of peptidyl tRNA. Product Information Product Information. Catalog Number Size Recombinant Human GSPT1/ERF3A Protein,GST&His Tag 96751ES25 25 μg 96751ES60 100 μg Product Properties Synonyms ERF3A,FLJ39067,GST1 Species Human Expression Sequence Phe294-Asp499,with C-terminal GST&His Accession P15170-1 Tag C-GST-His Tag Expression System Baculovirus-Insect Cells Molecular Weight 51 kDa (Reducing) Purity >85% as determined by SDS-PAGE Endotoxin
