The Hieff™ Plasmid Mini Kit adopts Hieff™ DNA Column P3 and a new solution system, and uses the conventional alkali cracking method to separate plasmid DNA. In the extraction process, toxic organic substances such as phenol and chloroform are not needed to be extracted, and time-consuming isopropanol or ethanol precipitation is not needed. The Hieff™ DNA Column P3 in the kit can specifically adsorb plasmid DNA, and does not adsorb protein, polysaccharide and other non-nucleic acid substances, so it is efficient, rapid and convenient. The extracted plasmid DNA has high purity and can be directly used in subsequent experiments such as DNA digestion, transformation and sequencing. Feature Wide Sample Range: Suitable for routine small-scale plasmid extraction; processes 2–6 mL of bacterial culture per prep. High DNA Purity: Free from protein and RNA contamination; DNA is ready for downstream applications such as digestion, transformation, and sequencing. High DNA Yield: Columns can bind up to 60 μg of plasmid DNA. Components Components No. Name 19001ES08 19001ES50 19001ES70 5 T 50 T 200 T Part I 19001-A RNase A (10 mg/mL) 15 µL 125 µL 500 µL Part II 19001-B RS Buffer P3* 1.5 mL 12.5 mL 50 mL 19001-C AC Buffer P3 500 µL 5 mL 20 mL 19001-D Hieff™ DNA Column P3 5 50 200 19001-E 2 mL Collection Tube P3 5 50 200 19001-F Lysis Buffer(LB Buffer P3) 1.5 mL 12.5 mL 50 mL 19001-G Binding Buffer(BD Buffer P3) 1.8 mL 17.5 mL 70 mL 19001-H Deproteinization Buffer(PL Buffer P3*) 1.6 mL 16 mL 63 mL 19001-I Wash Buffer* 1.3 mL 13 mL 50 mL 19001-J Elution Buffer 1 mL 10 mL 20 mL Application Plasmid Extraction Figures 1. High Yields Figure 1. Comparison of Plasmid DNA Yields from Equal Volumes of Bacterial CulturePlasmid yield: 19001 > Supplier M > Supplier F.
