IL-27 (interleukin 27) is a cytokine belonging to the IL-12 family, a heterodimeric cytokine consisting of EBI3 and p28 subunits.IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines.IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines.IL-27 is produced through the induction of the expression of the transcription factor T-bet and up-regulation of IL-12Rβ 2 to promote the differentiation of CD4+ T cells into the Th1 lineage, which in turn inhibits the differentiation and proliferation of Th2 and Th17.IL-27 is a member of the recently discovered family of proinflammatory and immunomodulatory cytokines IL-6/IL-12.IL-27 acts by binding to its receptors WSX-1 and gp130, which leads to the activation of the Jak/STAT signaling cascade, suggesting that IL-27 is involved in the regulation of immune processes. Recombinant Human IL-27 produced by HEK293 cells is a non-disulfide-linked heterodimeric protein consisting of the 209 amino acid-long EBI3 subunit and the 215 amino acid-long IL-27-p28 subunit, with a total sequence length of 424 amino acid residues. This product is available as Fluids, high purity, high activity, low endotoxin, NO labeling. Product Properties Synonyms Human IL-27, h-IL-27, rh-IL-27, recombinant IL-27, interleukin-27 Species Human Source HEK293 Ceslls Sequence IL-27 EBI-3(Arg21-Lys229),IL-27 p28(Phe29-Pro243) Accession Q14213.2 (IL-27EBI3) & AAM34498 (IL-27p28 Tag NO Predicted molecularmass 56.0 kDa Endotoxin 95%, determined by SDS-PAGE Activity Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC50 for this effect is 1-6 ng/mL Formulation Dissolved in sterile PBS buffer. Appearance Fluids Shipping and Storage -85~-65℃ storage, valid for 1 year after receipt. Avoid repeated freezing and thawing, centrifuge before opening the vial. It is recommended to aliquot for one experimental use, after aliquoting, freeze at -80℃. Figure Figure 1.Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC50 for this effect is 1-6 ng/mL. Figure 2.4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
