Product Properties Alternative Names IL30,IL-27A,p28,Interleukin-27 Species Human Expression Sequence Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A) Accession Q14213(IL-27B)&Q8NEV9(IL-27A) Tag C-His Expression System CHO Molecular Weight The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity ELISA Data: Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA . Reconstitution Method Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃, valid for 3 months.
