Nectin-4 is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90% amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively. Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with Nectin1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus. In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development. Specifications Synonyms EDSS1, LNIR, Nectin4, PRR4, NECTIN4, PVRL4 Uniprot No. Q96NY8 Source HEK293 Cells-derived Human Nectin-4 Protein, Gly32-Ser349 with hFc tag at the C-terminal Molecular Weight Approximately 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity SPR Data: Human Nectin-4, hFc Tag captured on Protein A chip, can bind Human Nectin-1,His Tag with an affinity constant of 0.17 μM as determined in a SPR assay (Biacore T200). Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -20~-80℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3-6 months, -20~-80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1. Avoid repeated freezing and thawing. 2. Please operate with lab coats and disposable gloves, for your safety. 3. This product is for research use only. Product Data HB220629
