Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis. Mature human Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain. Within the ECD, human Axl shares approximately 82% aa sequence identity with mouse and rat Axl. A short alternately spliced form of human Axl has a 9 aa deletion in the extracellular juxtamembrane region. Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain. Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation, macrophage and dendritic cell phagocytosis of apoptotic cells, NK cell development from hematopoietic progenitor cells, and in vivo angiogenesis. Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness. It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow. It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses. A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6. Specifications Synonyms Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1 Uniprot No. P30530.1 Source Recombinant Human Axl Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Ala26-Pro449. Molecular Weight Approximately 72.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human GAS6, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Axl, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。 It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
