CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, SLAMF7, and CD319, is a 65-75 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. Within the ECD, human CRACC shares 54% and 52% aa sequence identity with mouse and rat CRACC, respectively. There are seven known isoforms of CRACC which are distinguished by deletions and/or substitutions in both their ECD and cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells. Specifications Synonyms CD2 subset 1, CRACC, CD319, CS1, Novel Ly9, SLAMF7, 19A, FOAP-12 Uniprot No. Q9NQ25-1 Source HEK293 Cells-derived Human SLAMF7 / CRACC / CD319 Protein, Ser23-Met226 with hFc tag at the C-terminal Molecular Weight Approximately 49.2 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Human SLAMF7, hFc Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Anti-SLAMF7 Antibody, hFc Tag with the EC50 7.3 ng/mL determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -20~-80℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3-6 months, -20~-80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1. Avoid repeated freezing and thawing. 2. Please operate with lab coats and disposable gloves, for your safety. 3. This product is for research use only. Product Data
