Biotinylated Human IL-21 Protein, His-Avi Tag _ 93307ES

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Specifications Synonyms Za11; IL-21;Interleukin-21; Source Biotinylated Human IL-21 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus. It contains Gln32-S er162.[Accession | Q9HBE4-1] Molecular Weight The protein has a predicted MW of 18.30 kDa same as SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human IL-21R, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-21, His Tag with the EC50 of 2.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22 μm filtered solution in 20mM NaAc, 500mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 500mM NaCl (pH 5.5). Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Documents: Manual 93307_Manual_HB20260821 Show more