Canine BMPR1A/ALK-3 Protein, hFc Tag

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Product Properties Alternative Names BMPR1A, ALK-3, ALK3, ALK 3 Species Canine Expression Sequence Gln24-Arg152 Accession NP_001138622.1 Tag C-hFc Expression System HEK293 Molecula r Weight The protein has a predicted MW of 40.94 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【Cell Based Assay】Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is typically 0.5 - 2.5 μg/mL in the presence of 50 ng/mL of recombinant human BMP4. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months. Show more