Specification Source Biotinylated Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ser32-Ser355.[Accession | Q13641-1] Molecular Weight The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Anti-TPBG Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 43.3 ng/ml determined by ELISA. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Caution 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
