Human TPBG/5T4 Protein, hFc Tag

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Product Propertie Alternative Names M6P1, 5T4AG, WAIF1, TPBG, 5T4 Species Human Expression Sequence Ser32-Ser355 Accession Q13641 Tag C-hFc Expression System HEK293 Molecular Weigh t The protein has a predicted MW of 62.08 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human TPBG, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months. Show more