Recombinant Human IL-1R3/IL-1 RAcP Protein, His-Avi Tag

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Specifications Source Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ser21-Glu359.[Accession | Q9NPH3-1] Molecular Weight The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result. Source Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human IL-1R3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Show more