Recombinant Biotinylated Human VEGF-C/Flt4-L Protein, His-Avi Tag

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Synonyms Flt4-L; VEGFc;VEGFC; VEGF-C Source Biotinylated Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Thr103-Arg227. [Accession | Q6FH59] Molecular Weight The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Biotinylated Human VEGF-C, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0). Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Show more