Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) , mFc Tag

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Product Properties Alternative Names CD309, KDR, VEGFR, VEGFR2, VEGFR-21, FLK1, KRD1, Ly73 Species Human Expression Sequence Ala20-Glu764 Accession P35968-1 Tag C-mFc Expression Sy stem HEK293 Molecular Weight The protein has a predicted MW of 110 kDa. Due to glycosylation, the protein migrates to 135-160 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human VEGF165, No Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 0.14μg/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months. Show more