Mouse CD40 Ligand/TNFSF5 Protein, His Tag

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Product Properties Alternative Names CD40L, CD40LG, CD154, TNFSF5, TNFSF5IMD3, CD40LIGM, gp39, HIGM1, T-BAM, TRAP, IGM, IMD3, CD40 Ligand Species Mouse Expression Sequence Met112-L eu260 Accession P27548 Tag N-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 17.50 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human CD40, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 6.9ng/ml determined by ELISA . Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year.After resuspension, store under sterile conditions at -85 to -65°C. The product is valid for three months. Show more